Abandoned uranium mine-induced effects in caged roach : a multiparametric approach for the evaluation of in situ metal toxicity
Identifieur interne : 000422 ( Main/Exploration ); précédent : 000421; suivant : 000423Abandoned uranium mine-induced effects in caged roach : a multiparametric approach for the evaluation of in situ metal toxicity
Auteurs : Béatrice Gagnaire [France] ; Anne Bado-Nilles [France] ; Stéphane Betoulle [France] ; Rachid Amara [France] ; Elodie Kerambrun [France] ; Xavier Denier [France] ; Christophe Minier [France] ; Christelle Adam-Guillermin [France] ; A. Le Guernic [France] ; Wilfried Sanchez [France]Source :
Abstract
In the frame of the characterization of environmental risk assessment linked to abandoned uranium mines in the Limousin region (France) and in the context of the DEVIL program (Development and validation of fish biomarkers for the implementation of environmental legislation) led by INERIS (National institute for industry environment and risks), a study has been conducted on the effects of uranium mining releases on roach health status. The first part of the work was the search of potential sites for the study. Based on information from IRSN (National institute for radioprotection and nuclear safety) and from the owner of old mines, two private pounds were identified: one presenting uranium contamination, and one upstream the mining zone presenting no uranium in water. Roaches were therefore caged during one month on these two pounds. Biological sampling have been performed at the beginning of the experiment, and 2 and 4 weeks after, in order to measure several parameters: immune parameters followed by flow cytometry (phagocytosis, ROS production, apoptosis, lyosomes), genotoxicity (comet assay), oxidative stress (glutathione, lipoperoxidation), biotransformation (EROD, GST, ABC transporter), neurotoxicity (AChE), endocrine disruption (vitellogenin) and physiological parameters (ADN/ARN ratio, stomach contents, otoliths, condition index, sex ratio). Physico-chemical parameters of water and sediments (cations, anions, metals, radioactive elements) were also followed and bioaccumulation of metals in several organs (muscle, liver, kidney, gonad and gill) was measured. The results showed on the uranium contaminated site a water contamination of iron, aluminium, barium and manganese. Aluminium and iron were also present in the water of the uranium-free site. The sediments from the uranium contaminated site showed high levels of radioactive elements coming from the disintegration chain of uranium. Preliminary biological results indicated a stimulation of immune parameters, an increase of oxidative stress and a decrease of AChE in fish coming from the uranium contaminated site compared to the uranium-free site. The whole results, using integrated index, will allow the establishment of roach health status in the context of pluri-metallic mining release pollution. These data will strengthen the research for the improvement of methods and tools needed for the environmental risk assessment associated to radioactive substances in the environment.
Url:
Affiliations:
- France
- Champagne-Ardenne, Haute-Normandie, Région Normandie
- Le Havre, Reims
- Université de Reims Champagne-Ardenne, Université du Havre
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Le document en format XML
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59655 Villeneuve d'Ascq Cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lille1.fr/</ref></desc></org></tutelle><tutelle active="#struct-300278" type="direct"><org type="institution" xml:id="struct-300278" status="VALID"><orgName>Université du Littoral Côte d'Opale</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR8187" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Denier, Xavier" sort="Denier, Xavier" uniqKey="Denier X" first="Xavier" last="Denier">Xavier Denier</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-131790" status="VALID"><orgName>Laboratoire d'Ecotoxicologie et des Milieux Aquatiques</orgName><orgName type="acronym">LEMA</orgName><desc><address><addrLine>UFR de Sciences et Techniques, Le Havre</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lehavre.fr/ulh_services/Laboratoire-d-Ecotoxicologie.html</ref></desc><listRelation><relation name="EA3222" active="#struct-300317" type="direct"></relation></listRelation><tutelles><tutelle name="EA3222" active="#struct-300317" type="direct"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName></affiliation></author><author><name sortKey="Minier, Christophe" sort="Minier, Christophe" uniqKey="Minier C" first="Christophe" last="Minier">Christophe Minier</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-131790" status="VALID"><orgName>Laboratoire d'Ecotoxicologie et des Milieux Aquatiques</orgName><orgName type="acronym">LEMA</orgName><desc><address><addrLine>UFR de Sciences et Techniques, Le Havre</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lehavre.fr/ulh_services/Laboratoire-d-Ecotoxicologie.html</ref></desc><listRelation><relation name="EA3222" active="#struct-300317" type="direct"></relation></listRelation><tutelles><tutelle name="EA3222" active="#struct-300317" type="direct"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName></affiliation></author><author><name sortKey="Adam Guillermin, Christelle" sort="Adam Guillermin, Christelle" uniqKey="Adam Guillermin C" first="Christelle" last="Adam-Guillermin">Christelle Adam-Guillermin</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-126126" status="INCOMING"><orgName>Institut de Radioprotection et de Sûreté Nucléaire</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-321966" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-321966" type="direct"><org type="institution" xml:id="struct-321966" status="INCOMING"><orgName>Institut de Radioprotection et de Sûreté Nucléaire</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Le Guernic, A" sort="Le Guernic, A" uniqKey="Le Guernic A" first="A." last="Le Guernic">A. Le Guernic</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-126126" status="INCOMING"><orgName>Institut de Radioprotection et de Sûreté Nucléaire</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-321966" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-321966" type="direct"><org type="institution" xml:id="struct-321966" status="INCOMING"><orgName>Institut de Radioprotection et de Sûreté Nucléaire</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Sanchez, Wilfried" sort="Sanchez, Wilfried" uniqKey="Sanchez W" first="Wilfried" last="Sanchez">Wilfried Sanchez</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-3186" status="VALID"><orgName>Institut National de l'Environnement Industriel et des Risques</orgName><orgName type="acronym">INERIS</orgName><desc><address><country key="FR"></country></address><ref type="url">http://www.ineris.fr/</ref></desc><listRelation><relation active="#struct-300023" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300023" type="direct"><org type="institution" xml:id="struct-300023" status="VALID"><orgName>INERIS</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author></analytic></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the frame of the characterization of environmental risk assessment linked to abandoned uranium mines in the Limousin region (France) and in the context of the DEVIL program (Development and validation of fish biomarkers for the implementation of environmental legislation) led by INERIS (National institute for industry environment and risks), a study has been conducted on the effects of uranium mining releases on roach health status. The first part of the work was the search of potential sites for the study. Based on information from IRSN (National institute for radioprotection and nuclear safety) and from the owner of old mines, two private pounds were identified: one presenting uranium contamination, and one upstream the mining zone presenting no uranium in water. Roaches were therefore caged during one month on these two pounds. Biological sampling have been performed at the beginning of the experiment, and 2 and 4 weeks after, in order to measure several parameters: immune parameters followed by flow cytometry (phagocytosis, ROS production, apoptosis, lyosomes), genotoxicity (comet assay), oxidative stress (glutathione, lipoperoxidation), biotransformation (EROD, GST, ABC transporter), neurotoxicity (AChE), endocrine disruption (vitellogenin) and physiological parameters (ADN/ARN ratio, stomach contents, otoliths, condition index, sex ratio). Physico-chemical parameters of water and sediments (cations, anions, metals, radioactive elements) were also followed and bioaccumulation of metals in several organs (muscle, liver, kidney, gonad and gill) was measured. The results showed on the uranium contaminated site a water contamination of iron, aluminium, barium and manganese. Aluminium and iron were also present in the water of the uranium-free site. The sediments from the uranium contaminated site showed high levels of radioactive elements coming from the disintegration chain of uranium. Preliminary biological results indicated a stimulation of immune parameters, an increase of oxidative stress and a decrease of AChE in fish coming from the uranium contaminated site compared to the uranium-free site. The whole results, using integrated index, will allow the establishment of roach health status in the context of pluri-metallic mining release pollution. These data will strengthen the research for the improvement of methods and tools needed for the environmental risk assessment associated to radioactive substances in the environment.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Champagne-Ardenne</li><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li><li>Reims</li></settlement><orgName><li>Université de Reims Champagne-Ardenne</li><li>Université du Havre</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Gagnaire, Beatrice" sort="Gagnaire, Beatrice" uniqKey="Gagnaire B" first="Béatrice" last="Gagnaire">Béatrice Gagnaire</name></noRegion><name sortKey="Adam Guillermin, Christelle" sort="Adam Guillermin, Christelle" uniqKey="Adam Guillermin C" first="Christelle" last="Adam-Guillermin">Christelle Adam-Guillermin</name><name sortKey="Amara, Rachid" sort="Amara, Rachid" uniqKey="Amara R" first="Rachid" last="Amara">Rachid Amara</name><name sortKey="Bado Nilles, Anne" sort="Bado Nilles, Anne" uniqKey="Bado Nilles A" first="Anne" last="Bado-Nilles">Anne Bado-Nilles</name><name sortKey="Betoulle, Stephane" sort="Betoulle, Stephane" uniqKey="Betoulle S" first="Stéphane" last="Betoulle">Stéphane Betoulle</name><name sortKey="Denier, Xavier" sort="Denier, Xavier" uniqKey="Denier X" first="Xavier" last="Denier">Xavier Denier</name><name sortKey="Kerambrun, Elodie" sort="Kerambrun, Elodie" uniqKey="Kerambrun E" first="Elodie" last="Kerambrun">Elodie Kerambrun</name><name sortKey="Le Guernic, A" sort="Le Guernic, A" uniqKey="Le Guernic A" first="A." last="Le Guernic">A. Le Guernic</name><name sortKey="Minier, Christophe" sort="Minier, Christophe" uniqKey="Minier C" first="Christophe" last="Minier">Christophe Minier</name><name sortKey="Sanchez, Wilfried" sort="Sanchez, Wilfried" uniqKey="Sanchez W" first="Wilfried" last="Sanchez">Wilfried Sanchez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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